کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10971808 | 1106747 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The cellular protein expression of Foxp3 in lymphoid and non-lymphoid organs of Nile tilapia
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کلمات کلیدی
Forkhead domainFOXP3PBMCPHAFHDTregsPhytohemagglutininIHC - ایمونوهیستوشیمیimmunohistochemical - ایمونوهیستوشیمیImmunohistochemistry - ایمونوهیستوشیمیperipheral blood mononuclear cells - سلول های تک هسته ای خون محیطیcellular - سلولیRegulatory T cells - سلولهای تی تنظیمکنندهmajor histocompatibility complex - مجموعه سازگاری بافتی اصلیMHC - مجموعه سازگاری بافتی اصلیAntibody - پادتَن یا آنتیبادیforkhead box protein 3 - پروتکل جعبه جعبه 3
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In the present study, an antibody highly specific to the Nile tilapia (Oreochromis niloticus) Foxp3 was produced and characterized. Immunohistochemistry analysis indicates that Foxp3 was expressed in peripheral blood mononuclear cells (PBMC) and certain packed lymphocytes in particular, what's more, the percentage of Foxp3+ cells among PBMC was 5.7 ± 2.0% (n = 5) in healthy adults and could be significantly up-regulated after phytohemagglutinin (50 μg/ml) stimulation in vitro at 6, 12 and 24 h, respectively. In the lymphoid tissues, such as the thymus, spleen and head kidney, Foxp3 expression was observed mainly in lymphocyte-like cells. Surprisingly, in the non-lymphoid organ stomach, Foxp3 was detected in epithelial-like cells within the mucosa. Our study demonstrates for the first time that Foxp3 protein expression occurs not only in hematopoietic cells of lymphoid organ systems but also non-hematopoietic cells of non-lymphoid organ in lower vertebrates such as the fish tilapia. The conserved expression pattern of Foxp3 at the protein and cellular levels implies that it might have conserved functions from fish to mammals.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 45, Issue 2, August 2015, Pages 300-306
Journal: Fish & Shellfish Immunology - Volume 45, Issue 2, August 2015, Pages 300-306
نویسندگان
Cunxin Jia, Yujie Zhou, Xiaohuan Huang, Xi Peng, Linyan Liu, Linyan Zhou, Li Jin, Hongjuan Shi, Jing Wei, Deshou Wang,