A newly identified anti-lipopolysaccharide factor from the swimming crab Portunus trituberculatus with broad spectrum antimicrobial activity

► A new ALF isoform (PtALF6) from Portunus trituberculatus was cloned. ► PtALF6 shared the conserved structures with the reported ALFs. ► PtALF6 transcript was predominantly expressed in gill. ► The recombinant PtALF6 showed broad spectrum antimicrobial activity.