کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10973049 1108010 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of amino acid profile of mare milk produced in the highlands of the Kyrgyz Republic during the milking season
ترجمه فارسی عنوان
تعیین مشخصات اسید آمینه شیر مرغ تولید شده در مناطق بلند استان قرقیزستان در طول فصل شیردوشی
کلمات کلیدی
آمینو اسید، کروماتوگرافی مایع با کارایی بالا، شیر مادر فصل شیردوشی،
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی
This study was carried out to determine the influence of milking season on amino acid (AA) profile and chemical composition of milk samples from Kyrgyz native breed mares under traditional pasture conditions. Milk samples were collected monthly from May to August 2014 from mares grazing at 2,200 m above sea level. The AA composition was determined by precolumn derivatization with diethyl ethoxymethylenemalonate in HPLC and 18 AA were determined in mare milk. The analytical parameters of linearity (0.01-4 μg/mL), precision of the method (0.26-4.88% relative standard deviation), derivatization procedure (0.82-2.80% relative standard deviation), and instrument precision (0.08-1.00% relative standard deviation) were determined. The most abundant AA were glutamic acid (0.393-480 g/100 g of milk), leucine (0.192-0.230 g/100 g of milk), and lysine (0.192-0.230 g/100 g of milk). The amount of ornithine was small (0.002-0.015 g/100 g of milk). The percentages of essential AA in the protein of mare milk were 46, 46, 51, and 48% for May, June, July, and August, respectively, which demonstrate the high biological value of milk during the whole milking season.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Dairy Science - Volume 99, Issue 4, April 2016, Pages 2480-2487
نویسندگان
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