کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10973467 1108015 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fluorometric determination of free and total isocitrate in bovine milk
ترجمه فارسی عنوان
تعیین فلورومتری ایزو سیترات آزاد و کل در شیر گاو
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی
Isocitrate is an intermediate metabolite in the citric acid cycle found both inside the mitochondria as well as outside in the cytosolic shunt. Oxidation of isocitrate is believed to deliver large fractions of energy [i.e., reducing equivalents (NADPH) in the bovine udder] used for fatty acid and cholesterol synthesis. This study describes a new analytical method for determination of free and total isocitrate in bovine milk where time-consuming pretreatment of the sample is not necessary. Methods for estimation of both total isocitrate and free isocitrate are described, the difference being the esterified or even lactonized isocitrate. On average, 20% (6-27%) of cow milk isocitrate was esterified and free isocitrate correlated significantly with total isocitrate (r = 0.98). The present fluorometric determination correlated well with the traditional spectrophotometric determination of isocitrate. Milk samples from Danish Holstein cows (984) contained significantly less isocitrate than milk from Danish Jersey cows (760; i.e., 0.134 vs. 0.211 mmol/L). Isocitrate in milk is correlated to milk protein, fat, and citrate, and it is speculated, based on biochemistry, former studies, and the present, that isocitrate may reflect the energy situation in the mammary gland. The use of isocitrate as a biomarker of the energy status in the dairy cow is warranted.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Dairy Science - Volume 97, Issue 12, December 2014, Pages 7498-7504
نویسندگان
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