کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10974375 | 1108024 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Short communication: Predictive ability of Fourier-transform mid-infrared spectroscopy to assess CSN genotypes and detailed protein composition of buffalo milk
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Short communication: Predictive ability of Fourier-transform mid-infrared spectroscopy to assess CSN genotypes and detailed protein composition of buffalo milk Short communication: Predictive ability of Fourier-transform mid-infrared spectroscopy to assess CSN genotypes and detailed protein composition of buffalo milk](/preview/png/10974375.png)
چکیده انگلیسی
The aim of this work was to test the applicability of Fourier-transform mid-infrared spectroscopy (FT-MIR) for the prediction of the contents of casein (CN) and whey protein fractions in buffalo milk. Buffalo milk samples spectra were collected using a MilkoScan FT2 (Foss, Hillerød, Denmark) over the spectral range from 5,000 to 900 wavenumber à cmâ1. Contents of protein fractions, as well as CSN1S1 and CSN3 genotypes, were assessed by reversed phase HPLC. The highest coefficients of determination in cross-validation (1 â VR) were obtained for the contents (g/L of milk) of total protein and CN (1 â VR = 0.92), followed by the content of β-CN, total whey protein, and αS2-CN (1 â VR of 0.87, 0.77, and 0.63, respectively). Conversely, contents of αS1-CN, γ-CN, glycosylated-κ-CN, total κ-CN, and whey protein fractions were predicted with poor accuracy (1 â VR <0.51). When protein fractions were expressed as percentages to total protein, 1 â VR values were never greater than 0.61 (β-CN). Only 56 and 70% of the observations were correctly classified by discriminant analysis in each of 2 groups of CSN1S1 and CSN3 genotypes, respectively. Results showed that FT-MIR spectroscopy is not applicable when prediction of detailed milk protein composition with high accuracy is required. Predictions may play a role as indicator traits in selective breeding, if the genetic correlation between FT-MIR predictions and measures of milk protein composition are high enough and predictions of protein fraction contents are sufficiently independent from the predicted total protein content.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Dairy Science - Volume 98, Issue 9, September 2015, Pages 6583-6587
Journal: Journal of Dairy Science - Volume 98, Issue 9, September 2015, Pages 6583-6587
نویسندگان
V. Bonfatti, A. Cecchinato, P. Carnier,