Y-STR haplotype diversity among the Khandayat population of Odisha, India

We analyzed seventeen Y-chromosomal short tandem repeat (Y-STR) loci in a population sample of Khandayat community residing in Odisha, India in order to find out the haplotype diversity. Blood samples were collected from 136 unrelated male individuals and genomic DNA isolation was carried out by the standard organic extraction method followed by multiplex PCR amplification using an AmpFl STR Y-filer PCR amplification kit and genotyping. A total of 130 haplotypes were observed among the studied samples, out of which 126 were unique. Allele frequency and gene diversity were calculated. Haplotype diversity and discrimination capacity were found to be 0.999128 and 0.95588 respectively. Haplotypes of Khandayat population were compared with that of other Indian populations using AMOVA (analysis of molecular variance) tool to measure the genetic relatedness between various populations of India.