کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10979 | 711 | 2005 | 11 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Alteration of human neuroblastoma cell morphology and neurite extension with micropatterns Alteration of human neuroblastoma cell morphology and neurite extension with micropatterns](/preview/png/10979.png)
The spatial orientation of nerve cells plays a pivotal role in nerve regeneration. Here we report a new method for regulating neuronal cell morphology and guiding neurite extension on standard tissue culture dishes. Random copolymers of oligoethyleneglycol methacrylate and methacrylic acid [poly(OEGMA-co-MA)], microcontact printed on standard tissue culture dishes, resist cell attachment and remain intact in serum-containing medium for up to 2 weeks. Cell viability assay of SH-SY5Y cells demonstrated that poly(OEGMA-co-MA) on the substrate or in solution has no cytotoxic effect. When retinoic acid was added to SH-SY5Y cells, they extended neurites along the line patterns that are significantly longer than cells cultured on non-patterned culture dishes. The ability to guide neurite extension with micrometer precision is valuable for guiding directional growth of neurites and path finding of regenerating nerves.
Journal: Biomaterials - Volume 26, Issue 33, November 2005, Pages 6599–6609