کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10980163 | 1108069 | 2012 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of cooling and freezing storage on the stability of bioactive factors in human colostrum
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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چکیده انگلیسی
Breast milk constitutes the best form of newborn alimentation because of its nutritional and immunological properties. Banked human milk is stored at low temperature, which may produce losses of some bioactive milk components. During lactation, colostrum provides the requirements of the newborn during the first days of life. The aim of this study was to evaluate the effect of cooling storage at 4°C and freezing storage at â20°C and â80°C on bioactive factors in human colostrum. For this purpose, the content of IgA, growth factors such as epidermal growth factor, transforming growth factor (TGF)-β1 and TGF-β2, and some cytokines such as IL-6, IL-8, IL-10, and tumor necrosis factor (TNF)-α, and its type I receptor TNF-RI, were quantified. Some colostrum samples were stored for 6, 12, 24, and 48 h at 4°C and others were frozen at â20°C or â80°C for 6 and 12 mo. We quantified IgA, epidermal growth factor, TGF-β1, and TGF-β2 by indirect ELISA. Concentrations of IL-6, IL-10, and TNF-α cytokines, IL-8 chemokine, and TNF-RI were measured using the BD Cytometric Bead Array (BD Biosciences, Erembodegem, Belgium). Bioactive immunological factors measured in this study were retained in colostrum after cooling storage at 4°C for at least 48 h, with the exception of IL-10. None of the initial bioactive factor concentrations was modified after 6 mo of freezing storage at either â20°C or â80°C. However, freezing storage of colostrum at â20°C and â80°C for 12 mo produced a decrease in the concentrations of IgA, IL-8, and TGF-β1. In summary, colostrum can be stored at 4°C for up to 48 h or at â20°C or â80°C for at least 6 mo without losing its immunological properties. Future studies are necessary to develop quality assurance guidelines for the storage of colostrum in human milk banks, and to focus not only on the microbiological safety but also on the maintenance of the immunological properties of colostrum.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Dairy Science - Volume 95, Issue 5, May 2012, Pages 2319-2325
Journal: Journal of Dairy Science - Volume 95, Issue 5, May 2012, Pages 2319-2325
نویسندگان
C. RamÃrez-Santana, F.J. Pérez-Cano, C. AudÃ, M. Castell, M.G. Moretones, M.C. López-Sabater, C. Castellote, A. Franch,