کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10997804 | 1318699 | 2016 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Fast determination of multi-mycotoxins in corn by dispersive solid-phase extraction coupled with ultra-performance liquid chromatography with tandem quadrupole time-of-flight mass spectrometry
ترجمه فارسی عنوان
تعیین سریع چند میکروکوتوکسین در ذرت توسط استخراج جامدات فشرده شده همراه با کروماتوگرافی مایع فوق العاده عملکردی با طیف سنجی جرمی چهار ساعته
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی
A fast analytical method for the simultaneous determination of 9 mycotoxins, including aflatoxins (B1, B2, G1, and G2), fumonisins (B1, B2 and B3), zearalenone, and deoxynivalenol in corn using dispersive solid-phase extraction method and ultra-performance liquid chromatography coupled to tandem quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was developed and validated. Samples were extracted with acetonitrile-water (84:16, v:v, containing 1% acetic acid) using ultrasonic extraction. The extracts were purified with a dispersive SPE method using C18 as a cleaning agent. The final clear extracts were dried by nitrogen blowing and subsequently redissolved in methanol-water (5:5, v:v). The samples were then analyzed by UPLC-Q-TOF-MS with 0.1% formic acid in ammonium acetate-methanol as mobile phase. The mean recoveries were ranged from 68.0 to 120.0%, and the relative standard deviation (RSD) ranged from 0.18 to 6.29%. Limits of detections ranged from 0.05 to 50 μg kgâ1, and limits of quantification ranged from 0.1 to 200 μg kgâ1, which were below the legal limits set by the European Union for the legislated mycotoxins. The developed method was applied to 130 corn samples. Among the mycotoxins studied, aflatoxins B1 and fumonisins B1, B2 and B3 were the most predominant mycotoxins, and their concentrations were 0-593.12, 0-2.01Ã104, 0-6.94Ã103 and 0-3.05Ã103 μg kgâ1, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Integrative Agriculture - Volume 15, Issue 7, July 2016, Pages 1656-1666
Journal: Journal of Integrative Agriculture - Volume 15, Issue 7, July 2016, Pages 1656-1666
نویسندگان
Yan WANG, Yan-jie DONG, Zeng-mei LI, Li-gang DENG, Chang-ying GUO, Shu-qiu ZHANG, Da-peng LI, Shan-cang ZHAO,