کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11005645 | 1492020 | 2019 | 53 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rapid purification of lysozyme by mixed-mode adsorption chromatography in stirred fluidized bed
ترجمه فارسی عنوان
تصفیه سریع لیزوزیم توسط کروماتوگرافی جذب مخلوط حالت در بستر ذوب شده مایع
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The commercial STREAMLINE Direct HST adsorbent was employed to investigate the adsorption characteristics of lysozyme in complex chicken egg white (CEW). The effects of operating parameters, including adsorption pH, ionic strength, and hydrophobicity of liquids on binding capacity, were examined using a well-mixed contactor. To determine the elution strategy, the experiments were carried out in a small packed bed. The lysozyme was completely eluted by 0.5â¯M NaCl in 40â¯mM carbonate buffer (pH 12) at a high liquid velocity of 200â¯cm/h. The effect of rotating speed on fluidization characteristics was further investigated by using a stirred fluidized bed process. Smoother fluidization was observed when the rotating speed reached 200â¯rpm and lysozyme was directly recovered from highly viscous CEW in a single step with a high yield of 94.3% and a purification factor of 15.7.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 272, 30 January 2019, Pages 619-627
Journal: Food Chemistry - Volume 272, 30 January 2019, Pages 619-627
نویسندگان
Kuei-Hsiang Chen, Shin-Ying Chou, Yu-Kaung Chang,