کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
11016218 1777995 2018 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Antilipase activity guided fractionation of Vinca major
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Antilipase activity guided fractionation of Vinca major
چکیده انگلیسی
Methanolic extract of flowers of Vinca major (VFE) reduced the activity of porcine pancreatic lipase (PPL) which was reported in our previous study. To identify the antilipase molecule(s) in the VFE, the antilipase activity-guided fractionation for the purification of lipase inhibitory molecule(s) by Silica Gel and Sephadex G-50 column chromatography was attempted, and the final anti-lipase fraction was examined by ESI-MS and FT-IR spectroscopy. Two molecules namely, Majoridine and Akuammine were elucidated to be present in the flowers of V. major which possessed high PPL inhibition activity. Further, both molecules were looked in silico for their interactions with PPL and other important proteins of lipid metabolism using molecular docking studies. Majoridine and Akuammine demonstrated significant binding interactions with active site residues of PPL.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of King Saud University - Science - Volume 30, Issue 4, October 2018, Pages 433-439
نویسندگان
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