کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11024881 | 1701066 | 2019 | 35 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Optimization of fermentation medium for a newly isolated yeast strain (Zygosaccharomyces rouxii JM-C46) and evaluation of factors affecting biosynthesis of D-arabitol
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A newly isolated yeast strain, namely Zygosaccharomyces rouxii JM-C46, was reported to be promising for enhanced D-arabitol production from glucose. In this study, further efforts were made to optimize fermentation conditions for this strain through one-factor-at-a-time strategy considering seven important factors of shake flask fermentation. Subsequently, composition of fermentation medium was optimized using Box-Behnken design (BBD) of response surface methodology (RSM) including three major nitrogen providing ingredients, namely yeast extract, (NH4)2SO4 and peptone. Maximum D-arabitol concentration produced in one-factor-at-a-time experiments was found to be 72.69â¯g/L under the optimum conditions consisting of 200â¯g/L initial glucose, 100â¯ml medium, 5% (equivalent to 8.78â¯g/L) initial inoculum, temperature 30â¯Â°C, pH 5.0, fermentation time 96â¯h, and agitation rate 200â¯rpm. In the experiments of BBD, the predicted yield of D-arabitol varied between 67.73â¯g/L and 76.76â¯g/L, while the actual concentration of D-arabitol ranged from 68.02â¯g/L to 76.95â¯g/L. The optimum composition of medium F (final medium) contained d-glucose (200â¯g/L), yeast extract (10â¯g/L), (NH4)2SO4 (2â¯g/L) and peptone (7.5â¯g/L), which further increased D-arabitol production from 72.69â¯g/L to 76.32â¯g/L.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: LWT - Volume 99, January 2019, Pages 319-327
Journal: LWT - Volume 99, January 2019, Pages 319-327
نویسندگان
Qi Guo, H. Zabed, Huanhuan Zhang, Xu Wang, Junhua Yun, Guoyan Zhang, Miaomiao Yang, Wenjing Sun, Xianghui Qi,