کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11025836 | 1666509 | 2018 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Parallel testing increases detection of Mycobacterium bovis-infected African buffaloes (Syncerus caffer)
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کلمات کلیدی
PPDBM. bovisESAT-6CFP-10MGRBTBIGRAIFN-γKNPPBSPPDAELISA - تست الیزاBovine tuberculosis - سل گاویPhosphate buffered saline - فسفات بافر شورArc - قوسMycobacterium bovis - مایکوباکتریوم بویسHIP - مفصل ران یا مفصل هیپpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازKruger National Park - پارک ملی کروگرInterferon gamma - گاما اینترفرون
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The diagnosis of Mycobacterium bovis (M. bovis) infection in African buffaloes (Syncerus caffer) relies on detection of the cell-mediated immune response to M. bovis antigens using the single comparative intradermal tuberculin test (SCITT) or interferon gamma release assays (IGRAs). The aim of the present study was to determine whether parallel testing with the SCITT and an IGRA increases the number of M. bovis-infected buffaloes detected by these assays. Culture-confirmed animals (nâ=â71) tested during routine bovine tuberculosis (bTB) control programmes in Hluhluwe iMfolozi Park and Madikwe Game Reserve in South Africa, were used in this study. Results from 35 buffaloes tested using the SCITT and three Bovigam® IGRAs (cohort A) and 36 buffaloes tested using the SCITT, standard Bovigam® IGRA and Qiagen Cattletype IGRA (cohort B) were analysed. The parallel use of the SCITT with selected IGRAs was able to identify all animals in both cohorts. These findings are in agreement with cattle studies supporting the use of the SCITT and IGRAs in parallel to identify the greatest number of M. bovis-infected animals. The suggested parallel testing algorithm should be strategically applied to maximize detection of M. bovis infection in bTB-positive buffalo herds.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Immunology and Immunopathology - Volume 204, October 2018, Pages 40-43
Journal: Veterinary Immunology and Immunopathology - Volume 204, October 2018, Pages 40-43
نویسندگان
Netanya Bernitz, Wynand J. Goosen, Charlene Clarke, Tanya J. Kerr, Roxanne Higgitt, Eduard O. Roos, David V. Cooper, Robin M. Warren, Paul D. van Helden, Sven D.C. Parsons, Michele A. Miller,