کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11033774 | 1569758 | 2018 | 23 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular characterization of a novel ovodefensin gene in chickens
ترجمه فارسی عنوان
خصوصیات مولکولی ژن جدید اوودوفنسین در جوجه ها
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کلمات کلیدی
AMPORFRT-PCRTSBHDPphylogenetic analysis - تجزیه و تحلیل فیلوژنتیکrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدTryptone soya broth - سس سویا تریپتونAntimicrobial activity - فعالیت ضدمیکروبیopen reading frame - قاب خواندن بازLuria broth - لوریا بوثهRace - مسابقهreal-time reverse transcription polymerase chain reaction - واکنش زنجیره پلیمراز رونویسی معکوس در زمان واقعیhost defense peptide - پپتید دفاع میزبانHost defense peptides - پپتیدهای دفاع میزبانAntimicrobial peptide - پپتیدهای ضدمیکروبی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
چکیده انگلیسی
Host defense peptides (HDPs) represent a large group of diverse small peptides that play important roles in host defense and disease resistance. In vertebrates, one of the main types of HDPs belong to defensins, which are less than 100 amino acid residues and characterized by a highly conserved motif of cysteine residues. Recently, a subfamily of defensins, namely ovodefensins (OvoDs), has been identified in birds and reptiles. However, both their family members and evolutionary relationships remain unclear. In the present study, we cloned and characterized a novel gene namely OvoDBβ in chickens. Our results showed that the full length of chicken OvoDBβ mRNA contains 344â¯bp nucleotides and encodes a 61-amino acid protein. We further revealed that the mRNA of OvoDBβ is abundant in the oviduct of laying hens but absent in many other tissues. Additionally, sequences comparison and analyses suggested that OvoDBβ is orthologous to the gene previously known as zebra finch OvoDB1, albeit it might exhibit specific structures. Furthermore, both OvoDBα and OvoDBβ were existent in the genome of each bird, implying that two types of OvoDBs sharing same cysteine motif have already emerged before the species divergence. More importantly, recombinant OvoDBβ mature peptide exerted antibacterial activity against Escherischia coli (CICC23657 strain) in vitro. These results collectively indicated that the putative sequence, namely chicken OvoDBβ, is a function gene with potential antimicrobial property. Discovery and function characterization of novel HDP genes may help us develop novel antimicrobial agents in the future.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 678, 15 December 2018, Pages 233-240
Journal: Gene - Volume 678, 15 December 2018, Pages 233-240
نویسندگان
Lin-tian Yu, Ying-Ping Xiao, Jing-Jing Li, Jin-Shan Ran, Ling-Qian Yin, Yi-Ping Liu, Long Zhang,