کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11074 | 716 | 2005 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Cellular and molecular events during chondrogenesis of human mesenchymal stromal cells grown in a three-dimensional hyaluronan based scaffold Cellular and molecular events during chondrogenesis of human mesenchymal stromal cells grown in a three-dimensional hyaluronan based scaffold](/preview/png/11074.png)
Mesenchymal stromal cells (MSCs) seem to be a good alternative to chondrocytes for cartilage regeneration. To obtain new information on the sequence of cellular and molecular events during in vitro chondrogenic differentiation we analysed MSCs on a widely used hyaluronic acid biomaterial (Hyaff®-11). Cellular differentiation was induced using two different concentrations of TGFβ1TGFβ1 (10 and 20 ng/ml) and the process was analysed at different time points (24 h, and 7, 14, 21 and 28 days) using techniques of light and electron microscopy, real-time PCR and immunohistochemistry. We found that without TGFβTGFβ MSCs did not survive while in the presence of TGFβTGFβ the cells significantly proliferated from day 7 until day 28. Light and electron microscopy showed that TGFβTGFβ at 20 ng/ml better induced the formation of cartilage-like tissue. Real-time PCR showed an increased expression of collagen type II, IX and aggrecan associated to a down-regulation of collagen type I. Immunohistochemical analysis confirmed that collagen type I was down-modulated while collagen type II increased from day 14 to day 28.These data clearly showed that higher concentrations of TGFβTGFβ (20 ng/ml) induce chondrogenesis of MSCs on Hyaff®-11 scaffold better than 10 ng/ml of TGFβTGFβ. This process is characterized by a sequence of cellular and molecular events that deal with the in vitro formation of a cartilage-like tissue.
Journal: Biomaterials - Volume 26, Issue 28, October 2005, Pages 5677–5686