کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11259799 | 1840435 | 2018 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Deoxynivalenol induces toxicity and apoptosis in piglet hippocampal nerve cells via the MAPK signaling pathway
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Deoxynivalenol (DON) is a mycotoxin capable of producing a variety of toxic effects in human and animals. In this study, the effect of DON treatment on cytotoxicity and apoptotic pathways in piglet hippocampal nerve cells (PHNCs) was determined. The effects of DON on cellular morphology, cell activity, lactate dehydrogenase (LDH) release, the protein expression of mitogen-activated protein kinase (MAPK) pathway, and the relative expression of key genes related to apoptosis were evaluated. The results indicated that DON significantly inhibited cellular viability and promoted the release of LDH by damaging the membrane integrity of PHNCs, however, the cellular viability was increased and LDH leakage rate were decreased after adding MAPK inhibitors. DON induced PHNCs apoptosis and phosphorylation of MAPK pathway proteins dose-dependently. The ratios of phospho p-JNK/JNK and p-p38/p38 significantly increased with the increase of DON concentration, while the p-ERK/ERK ratio significantly decreased. In addition, DON upregulated the BAX mRNA level, and downregulated the BCL2 mRNA level. Pre-incubation with inhibitors of JNK (SP600125) and p38 (SB202190) significantly decreases the BAX/BCL2 ratio. However, pre-incubation with the inhibitor of ERK (U0126), significantly increased the BAX/BCL2 ratio. These data demonstrated that DON induces toxic effects and apoptosis in PHNCs via the MAPK signaling pathway.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicon - Volume 155, 1 December 2018, Pages 1-8
Journal: Toxicon - Volume 155, 1 December 2018, Pages 1-8
نویسندگان
Xichun Wang, Mengxue Fan, Xiaoyan Chu, Yafei Zhang, Sajid ur Rahman, Yunjing Jiang, Xiaofang Chen, Dianfeng Zhu, Shibin Feng, Yu Li, Jinjie Wu,