کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
11511 746 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The MR tracking of transplanted ATDC5 cells using fluorinated poly-l-lysine-CF3
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
The MR tracking of transplanted ATDC5 cells using fluorinated poly-l-lysine-CF3
چکیده انگلیسی

Magnetic resonance (MR) imaging using super-paramagnetic iron oxides (SPIOs) is a powerful tool to monitor transplanted cells in living animals. However, since SPIOs are negative contrast agents it is difficult to track transplanted cells in bone and cartilage that originally display low signals. In this study, we examined the feasibility of tracking with fluorescein isothiocyanate (FITC)-labeled poly-l-lysine-CF3 (PLK-CF3) using mouse ATDC5 cells, a stem cell line of bone and cartilage cells. FITC-labeled PLK-CF3 was easily internalized by ATDC5 cells by adding it into culture medium. No acute or long-term toxicities were seen at less than 160 μg/ml. Labeled cells transplanted into the cranial bone of mice were detected for at least 7 days by MR images. FITC-labeled PLK-CF3 is a useful positive contrast agent for MR tracking in bone and cartilage.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 28, Issue 3, January 2007, Pages 434–440
نویسندگان
, , , , , , , ,