کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1173058 1491350 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of neuraminidase detection using gold nanoparticles boron-doped diamond electrodes
ترجمه فارسی عنوان
توسعه تشخیص نورامینیداز با استفاده از الکترودهای الماس بور با استفاده از نانوذرات طلا
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی

Gold nanoparticles-modified boron-doped diamond (AuNPs–BDD) electrodes, which were prepared with a self-assembly deposition of AuNPs at amine-terminated boron-doped diamond, were examined for voltammetric detection of neuraminidase (NA). The detection method was performed based on the difference of electrochemical responses of zanamivir at gold surface before and after the reaction with NA in phosphate buffer solution (PBS, pH 5.5). A linear calibration curve for zanamivir in 0.1 M PBS in the absence of NA was achieved in the concentration range of 1 × 10−6 to 1 × 10−5 M (R2 = 0.99) with an estimated limit of detection (LOD) of 2.29 × 10−6 M. Furthermore, using its reaction with 1.00 × 10−5 M zanamivir, a linear calibration curve of NA can be obtained in the concentration range of 0–12 mU (R2 = 0.99) with an estimated LOD of 0.12 mU. High reproducibility was shown with a relative standard deviation (RSD) of 1.14% (n = 30). These performances could be maintained when the detection was performed in mucin matrix. Comparison performed using gold-modified BDD (Au–BDD) electrodes suggested that the good performance of the detection method is due to the stability of the gold particles position at the BDD surface.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 497, 15 March 2016, Pages 68–75
نویسندگان
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