کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1173335 | 961668 | 2011 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Optimal design for the dose–response screening of tight-binding enzyme inhibitors Optimal design for the dose–response screening of tight-binding enzyme inhibitors](/preview/png/1173335.png)
Optimal experimental designs for the dose–response screening of enzyme inhibitors were studied within the framework of the Box–Lucas theory. If the enzyme concentration E is considered as a fixed constant, an exact two-point D -optimal design consists of a pair of inhibitor concentrations equal to I1=0I1=0 and I2=E+KI2=E+K, where K is the apparent inhibition constant. If the enzyme concentration is treated as an adjustable parameter, an empirical three-point D -optimal design consists of three inhibitor concentrations equal to I1=0I1=0, I2=E+3KI2=E+3K, and I3=0.7EI3=0.7E. These results were applied to design optimized, irregularly spaced concentration series for routine inhibitor screening. A heuristic Monte Carlo simulation study confirmed that the optimized dilution series is significantly more efficient than the classic series characterized by a constant dilution ratio. An online calculator to create optimized dilution series is freely available at http://www.biokin.com/design/.
Journal: Analytical Biochemistry - Volume 419, Issue 2, 15 December 2011, Pages 117–122