کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1173398 1491398 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
One-step homogeneous non-stripping chemiluminescence metal immunoassay based on catalytic activity of gold nanoparticles
ترجمه فارسی عنوان
ایمونوفیزمالی فلز کرومولومینسانس بدون سایز یک مرحله ای بر اساس فعالیت کاتالیزوری نانوذرات طلا
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی

The catalytic activity of gold nanoparticles (AuNPs) on a luminol–H2O2 chemiluminescence (CL) system is found to be greatly enhanced after its crosslinking aggregation induced by immunoreaction. Based on this observation, a one-step homogeneous non-stripping CL metalloimmunoassay was designed. In the presence of corresponding antigen (Ag), the immunoreaction caused the aggregation of antibody (Ab)-modified AuNPs, and these crosslinking aggregated AuNPs could catalyze luminol–H2O2 CL reaction to produce a much stronger CL signal than dispersed Ab-modified AuNPs. The assay, including immunoreaction and detection, can be accomplished in homogeneous solution. In the assay, no tedious and strict stripping of metal nanoparticles, difficult synthesis of labels, multiple steps of immunoreactions and washings, and complicated magnetic separation process were required. The detection limit of human immunoglobulin G (IgG, 3σ) was estimated to be as low as 3.2 × 10−11 g ml−1. The sensitivity was increased by two orders of magnitude over that of other AuNP-based CL immunoassay. The current CL metalloimmunoassay offers the advantages of being simple, cheap, rapid, and sensitive.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 449, 15 March 2014, Pages 1–8
نویسندگان
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