کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1175660 961811 2009 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High-throughput assays probing protein–RNA interactions of eukaryotic translation initiation factors
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
High-throughput assays probing protein–RNA interactions of eukaryotic translation initiation factors
چکیده انگلیسی

Protein–RNA interactions are involved in all facets of RNA biology. The identification of small molecules that selectively block such bimolecular interactions could provide insight into previously unexplored steps of gene regulation. Such is the case for regulation of eukaryotic protein synthesis where interactions between messenger RNA (mRNA) and several eukaryotic initiation factors govern the recruitment of 40S ribosomes (and associated factors) to mRNA templates during the initiation phase. We have designed simple fluorescence polarization-based high-throughput screening assays that query the binding of several translation factors to RNA and found that the mixed inhibitor p-chloromercuribenzoate interferes with poly(A) binding protein–RNA interaction.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 384, Issue 1, 1 January 2009, Pages 180–188
نویسندگان
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