کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1176048 961829 2006 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Optimal Sox-based fluorescent chemosensor design for serine/threonine protein kinases
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Optimal Sox-based fluorescent chemosensor design for serine/threonine protein kinases
چکیده انگلیسی

Fluorescent chemosensors of protein kinase activity provide a continuous, high-throughput sensing format for the study of the roles of these enzymes, which are crucial for regulating cellular function. Specifically, chemosensors using the nonnatural amino acid, Sox, and physiological Mg2+ levels report phosphorylation with dramatic fluorescence changes that are amenable to real-time and high-throughput analysis. In this article, we report 15 probes for a total of six distinct serine/threonine kinases with large fluorescence increases and good reactivity toward the target kinase. The sensing mechanism is detailed, and the optimal sensing motif is determined. These versatile and powerful sensors provide tools for researchers studying the roles of the targeted kinases in signal transduction, and the design principles provide guidelines for the generation of future fluorescent chemosensors for any serine/threonine kinase.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 352, Issue 2, 15 May 2006, Pages 198–207
نویسندگان
, , ,