کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1177120 961947 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Formation and biliary excretion of glutathione conjugates of bile acids in the rat as shown by liquid chromatography/electrospray ionization–linear ion trap mass spectrometry
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Formation and biliary excretion of glutathione conjugates of bile acids in the rat as shown by liquid chromatography/electrospray ionization–linear ion trap mass spectrometry
چکیده انگلیسی

Acyl-adenylates and acyl-CoA thioesters of bile acids (BAs) are reactive acyl-linked metabolites that have been shown to undergo transacylation-type reactions with the thiol group of glutathione (GSH), leading to the formation of thioester-linked GSH conjugates. In the current study, we examined the transformation of cholyl-adenylate (CA–AMP) and cholyl-coenzyme A thioester (CA–CoA) into a cholyl-S-acyl GSH (CA–GSH) conjugate by rat hepatic glutathione S-transferase (GST). The reaction product was analyzed by liquid chromatography (LC)/electrospray ionization (ESI)–linear ion trap mass spectrometry (MS). The GST-catalyzed formation of CA–GSH occurred with both CA–AMP and CA–CoA. Ursodeoxycholic acid, lithocholic acid, and 2,2,4,4-2H4-labeled lithocholic acid were administered orally to biliary fistula rats, and their corresponding GSH conjugates were identified in bile by LC/ESI–MS2. These in vitro and in vivo studies confirm a new mode of BA conjugation in which BAs are transformed into their GSH conjugates via their acyl-linked intermediary metabolites by the catalytic action of GST in the liver, and the GSH conjugates are then excreted into the bile.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 384, Issue 2, 15 January 2009, Pages 224–230
نویسندگان
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