کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1177507 962021 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electrochemical DNA biosensor for the study of ciprofloxacin–DNA interaction
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Electrochemical DNA biosensor for the study of ciprofloxacin–DNA interaction
چکیده انگلیسی

The interaction of ciprofloxacin with DNA was studied by using an electrochemical DNA biosensor. The binding mechanism of ciprofloxacin was elucidated by using constant current potentiometry and differential pulse voltammetry at DNA-modified glassy carbon electrode. The decrease in the guanine oxidation peak area or peak current at +0.9 V was used as an indicator for the interaction mechanism in 0.2 M acetate buffer (pH 5). The binding constant (K) values obtained were 1.33 ± 0.02 × 104 and 1.32 ± 0.08 × 104 M−1 with constant current potentiometry and differential pulse voltammetry, respectively. A linear dependence of the guanine peak area or peak currents was observed in the range of 40–80 μM ciprofloxacin, with a detection limit of 24 μM with r = 0.995 and 9 μM with r = 0.999 by using constant current potentiometry and differential pulse voltammetry, respectively. Moreover, the influence of sodium and calcium ions was also studied to elucidate the mechanism of ciprofloxacin–DNA interaction at different solution conditions, and this proved to be helpful in understanding the ciprofloxacin–DNA interaction.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 354, Issue 1, 1 July 2006, Pages 28–34
نویسندگان
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