Novel analytical procedure using a combination of hollow fiber supported liquid membrane and dispersive liquid–liquid microextraction for the determination of aflatoxins in soybean juice by high performance liquid chromatography – Fluorescence detector

• High sensitive analysis of AFLs in soybean juices samples at μg L−1 levels.
• Hollow fiber coupled with dispersive liquid–liquid microextraction.
• Extraction free of chlorinated solvents and less than 500 μL of another solvents.
• Great potential for automation and application for other analytes and samples.

This study describes a combination between hollow fiber membrane and dispersive liquid–liquid microextraction for determination of aflatoxins in soybean juice by HPLC. The main advantage of this approach is the use of non-chlorinated solvent and small amounts of organic solvents. The optimum extraction conditions were 1-octanol as immobilized solvent; toluene and acetone at 1:5 ratio as extraction and disperser solvents (100 μL), NaCl at 2% of the sample volume and extraction time of 60 min. The optimal condition for the liquid desorption was 150 μL acetonitrile:water (50:50 v/v) and desorption time of 20 min. The linear range varied from 0.03 to 21 μg L−1, with R2 coefficients ranging from 0.9940 to 0.9995. The limits of detection and quantification ranged from 0.01 μg L−1 to 0.03 μg L−1 and from 0.03 μg L−1 to 0.1 μg L−1, respectively. Recovery tests ranged from 72% to 117% and accuracy between 12% and 18%.