کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1184892 | 1492151 | 2013 | 9 صفحه PDF | دانلود رایگان |
Saponins from the roots of Platycodon grandiflorum (Changkil saponins, CKS) have antioxidant and hepatoprotective properties. This study investigated the effects of CKS on AMP-activated protein kinase (AMPK) activation and hepatic lipogenesis in HepG2 cells. CKS suppressed high-glucose-induced lipid accumulation and inhibited high-glucose-induced fatty acid synthase (FAS) and sterol regulatory element binding protein-1c (SREBP-1c) expression in HepG2 cells. Moreover, the use of a pharmacological AMPK inhibitor revealed that AMPK is essential for the suppression of SREBP-1c expression in CKS-treated cells. Finally, the activation of calcium/calmodulin-dependent kinase kinase β (CaMKKβ) and SIRT1 was necessary for CKS-enhanced activation of AMPK. These results indicate that CKS prevents lipid accumulation in HepG2 cells by blocking the expression of SREBP-1c and FAS through SIRT1 and CaMKKβ/AMPK activation. Using CKS to target AMPK activation may provide a promising approach for the prevention lipogenesis.
► Saponins from Platycodon grandiflorum (CKS) suppressed high-glucose-induced lipid accumulation in HepG2 cells.
► CKS inhibited high-glucose-induced FAS and SREBP-1c expression in HepG2 cells.
► CKS promotes AMPKα phosphorylation in HepG2 cells.
► CKS-induced activation of AMPKα is regulated by CaMKKβ and SIRT1.
Journal: Food Chemistry - Volume 140, Issues 1–2, 1–15 September 2013, Pages 115–123