کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1185115 1492080 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Preparative separation of grape skin polyphenols by high-speed counter-current chromatography
ترجمه فارسی عنوان
جداسازی آماده سازی پلی فنول پوست انگور با کروماتوگرافی با سرعت بالا ضد جریان
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• Grape skin polyphenols were first separated according to increasing polarity by HSCCC.
• A series of grape skin polyphenols not commercially available were largely prepared.
• The proposed method would be applied to prepare other plant polyphenols.

To develop an efficient method for large preparation of various individual polyphenols from white grape skins (Fernão Pires; Vitis vinifera) by preparative high-speed counter-current chromatography (HSCCC) and preparative-HPLC, an optimized preparative HSCCC condition with two-phase solvent system composed of Hex-EtOAc-H2O (1:50:50, v/v) was used to separate grape skin polyphenols into various fractions. Both the tail-head and head-tail elution modes were used with a flow rate of 3.0 ml/min and a rotary speed of 950 rpm. Afterwards, a preparative-HPLC separation was applied to isolate individual polyphenols in each of the fractions from HSCCC. Total of 7 fractions (Fraction A to G) were obtained from grape skin extract by HSCCC. After preparative-HPLC isolation, fifteen individual compounds were obtained, most of which presented high yields and purity (all over 90%). The HSCCC method followed with preparative-HPLC appeared to be convenient and economical, constituting an efficient strategy for the isolation of grape skin polyphenols.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 212, 1 December 2016, Pages 712–721
نویسندگان
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