کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1185416 963399 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Free radical scavenging activity and immunomodulatory effect of Stachytarpheta angustifolia leaf extract
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Free radical scavenging activity and immunomodulatory effect of Stachytarpheta angustifolia leaf extract
چکیده انگلیسی

Plant extracts with antioxidant activity could also have immunomodulatory ability. The free radical scavenging activity of an ethanol extract of the leaves of Stachytarpheta angustifolia   was assessed by measuring its capability for scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anion radical (O2-), hydroxyl radical (OH), nitric oxide radicals (NO), as well as its ability to inhibit lipid peroxidation, using appropriate assay systems. The extract was also assessed for its ability to decrease the phenotypic expression of the immune activation markers CD38 and CD69. This extract showed a potent antioxidant activity in the DPPH radical-scavenging assay (EC50 = 9.65 μg/ml), significantly inhibited OH radical (IC50 = 99.43 μg/ml), O2- anion radical (IC50 = 64.68 μg/ml), non-enzymatic lipid peroxidation (IC50 = 282.91 μg/ml) and accumulation of nitrite in vitro. Ex vivo   the extract inhibited phorbol myristate acetate (PMA)-induced production of superoxide anion (O2-), and also exhibited a dose-dependent reduction in the levels of the immune activation marker CD38 and CD69 on phytohemagglutinin A (PHA)-stimulated human peripheral blood mononuclear cells (PBMC). The observed antioxidant activity and immunomodulatory potentials of the extract suggest that it could impart health benefits when consumed. However, further investigation to verify its effect in vivo is warranted.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 119, Issue 4, 15 April 2010, Pages 1409–1416
نویسندگان
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