Stability indicating HPLC–UV method for detection of curcumin in Curcuma longa extract and emulsion formulation

• Determination of curcumin in Curcuma longa extract and emulsion using HPLC method.
• System suitability studies were performed.
• Stress degradation studies of curcumin in emulsion formulation.

A stability-indicating HPLC–UV method for the determination of curcumin in Curcuma longa extract and emulsion was developed. The system suitability parameters, theoretical plates (N), tailing factor (T), capacity factor (K′), height equivalent of a theoretical plate (H) and resolution (Rs) were calculated. Stress degradation studies (acid, base, oxidation, heat and UV light) of curcumin were performed in emulsion. It was found that N > 6500, T < 1.1, K′ was 2.68–3.75, HETP about 37 and Rs was 1.8. The method was linear from 2 to 200 μg/mL with a correlation coefficient of 0.9998. The intra-day precision and accuracy for curcumin were ⩽0.87% and ⩽2.0%, while the inter-day precision and accuracy values were ⩽2.1% and ⩽−1.92. Curcumin degraded in emulsion under acid, alkali and UV light. In conclusion, the stability-indicating method could be employed to determine curcumin in bulk and emulsions.