کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1186289 963432 2010 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Preparation of eicosapentaenoic acid concentrates from sardine oil by Bacillus circulans lipase
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Preparation of eicosapentaenoic acid concentrates from sardine oil by Bacillus circulans lipase
چکیده انگلیسی

An extracellular lipase derived from Bacillus circulans, isolated from marine macroalga, Turbinaraia conoides, was used to prepare n-3 polyunsaturated fatty acid (PUFA) concentrates from sardine oil triglycerides. The enzyme was purified 132-fold with specific activity of 386 LU/mg. The purified lipase was able to enrich sardine oil with 37.7 ± 1.98% 20:5n-3 and 5.11 ± 0.14% 18:3n-3 in the triglyceride fraction after 3 h of hydrolysis. Lower hydrophobic constants of n-3 fatty acids (18:3n-3logP = 5.65; 20:5n-3logP = 5.85, respectively) than n-6 (20:4n-6logP = 6.16) resulted in higher hydrolytic resistance of the former toward lipase, leading to their enrichment in the triglyceride fraction. Lipase-catalysed hydrolysis of sardine oil for 3 h, followed by urea complexation, provided free fatty acids containing 51.3 ± 4.65% 20:5n-3. The purified methyl ester of 20:5n-3 (68.29 ± 2.15%) from the urea concentrate was attained by chromatography on argentated neutral alumina.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 120, Issue 2, 15 May 2010, Pages 433–442
نویسندگان
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