کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1186800 963447 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electrochemical glucose biosensing by pyranose oxidase immobilized in gold nanoparticle-polyaniline/AgCl/gelatin nanocomposite matrix
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Electrochemical glucose biosensing by pyranose oxidase immobilized in gold nanoparticle-polyaniline/AgCl/gelatin nanocomposite matrix
چکیده انگلیسی

A novel pyranose oxidase (PyOx) biosensor based on gold nanoparticles (AuNPs)–polyaniline(PANI)/AgCl/gelatin nanocomposite has been developed for the glucose detection. PyOx was immobilized on the surface of glassy carbon electrode (GCE) via the nanocomposite matrix. The electrode surface was imaged by scanning electron microscopy (SEM). Amperometric detection of the consumed oxygen during the enzymatic reaction was monitored at −0.7 V. After optimization studies, analytical characterization of the biosensor was carried out. The linear response of the AuNPs–AgCl/PANI/gelatin modified PyOx biosensor is found to be from 0.05 to 0.75 mM glucose with the equation of y = 2.043x + 0.253; R2 = 0.993. Finally, proposed biosensor was used to analyze glucose content in real samples. Obtained data from the biosensing system was compared with a commercial enzyme assay kit based on spectrophotometric Trinder reaction as a reference method.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 119, Issue 1, 1 March 2010, Pages 380–385
نویسندگان
, , , ,