Comprehensive evaluation of antioxidant activity for various substances with 5-axe cobweb chart

Antioxidant activities of various substances against peroxynitrite and peroxyl radical generated from 2,2′-azobis (2-methylpropionamidine) dihydrochloride (AAPH) were evaluated by the previously proposed method [Terashima, M., Nakatani, I., Harima, A., Nakamura, S., & Shiiba M. (2007). New method to evaluate water-soluble antioxidant activity based on protein structure change. Journal of Agricultural and Food Chemistry, 55(1), 165–169] using myoglobin as a probe. Activities of several antioxidants against five different reactive oxygen species, DPPH, hypochlorite ion, hydroxyl radical, peroxynitrite, and peroxyl radical, were characterised comprehensively by 5-axe cobweb charts. For example, Trolox, ascorbic acid, and ferulic acid show similar activities against DPPH radicals (7–10 mM VC), the shapes of the 5-axe cobweb charts are different from each others. This clear-cut expression would be useful to predict probable physiological effects of various antioxidants and to compare quality of antioxidants contained in food materials and food products.