کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1187293 963459 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of aflatoxins in animal feeds by HPLC with multifunctional column clean-up
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of aflatoxins in animal feeds by HPLC with multifunctional column clean-up
چکیده انگلیسی

A reversed-phase HPLC method with fluorescence detection for the determination of the aflatoxins B1, B2, G1 and G2 in 42 animal feeds, comprising corn (16), soya bean meal (8), mixed meal (13), sunflower, wheat, canola, palm kernel, copra meals (1 each) was carried out. The samples were first extracted using acetonitrile:water (9:1), and was further cleaned-up using a multifunctional column. Optimum conditions for the extraction and chromatographic separation were investigated. By adopting an isocratic chromatographic system using a mobile phase comprising acetonitrile:methanol:water (8:27:65, v/v/v), the separation of the four aflatoxins was possible within 30 min. Recoveries for aflatoxins B1, B2, G1 and G2 were 98 ± 0.7%, 95 ± 1.0%, 94 ± 3.6% and 97 ± 4.3%, respectively. The results show that eight samples (19%) were contaminated with aflatoxins, ranging from 6.5 to 101.9 ng g−1. Total aflatoxin levels in three samples exceed the legal limits of many countries of 20 ng g−1.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 118, Issue 3, 1 February 2010, Pages 882–886
نویسندگان
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