کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1187375 | 963462 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel visceral excitatory neuropeptide from the brain tissue of cloudy dogfish (Scyliorhinus torazame)
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A visceral excitatory neuropeptide with a molecular weight of 1563Â Da was isolated from the brain extracts of cloudy dogfish (Scyliorhinus torazame) discarded as a fishery by-product. Fish hindgut was employed to carry out physiological assay for its visceral excitation. During consecutive purification using C18 cartridge and high-performance liquid chromatography (HPLC), a neuropeptide (DF-2) was isolated from neuronal cell extracts of dogfish brain tissue and clearly exhibited potential to exert visceral excitatory effect on hindgut of dogfish. The primary structure of DF-2 established by ESI-Q-TOF tandem mass was LESLVYEQLWPWamide. The results of database search provided evidence for this peptide sequence to be novel. In visceral excitatory assay using myography, the threshold concentration of DF-2 required for the changes in spontaneous contraction of cloudy dogfish hindgut was found to be 10â6Â M. Therefore, this study suggests that DF-2 isolated from brain of elasmobranches could be one of the sequences, which regulates spontaneous visceral contractions in dogfish digestive duct.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 115, Issue 4, 15 August 2009, Pages 1306-1311
Journal: Food Chemistry - Volume 115, Issue 4, 15 August 2009, Pages 1306-1311
نویسندگان
Young-Sook Cho, Won-Kyo Jung, Sang-Hoon Lee, Eresha Mendis, Se-Kwon Kim,