کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1187411 | 963462 | 2009 | 7 صفحه PDF | دانلود رایگان |

Atmospheric pressure photoionization–tandem mass spectrometry (APPI–MS/MS) method has been developed for quantitative determination of Sudan I to IV dyes. This study demonstrates the applicability of a simple isocratic normal phase HPLC method using isopropanol (0.3%) in n-hexane as the mobile phase for the separation of these dyes. A simple extraction procedure using n-hexane has been applied for the extraction of these dyes from spiked samples of chilli powder and tomato sauce. The quantitative determination of Sudan I to IV is obtained from the spiked tomato sauce and chilli powder samples by external standard method under single reaction monitoring (SRM) mode. The study includes a detailed investigation on LOD, LOQ, linearity and recovery of Sudan I to IV dyes. The LOD ranged from 5–18 μg/l and LOQ ranged from 10–24 μg/l. The present method can be a powerful analytical tool for the simultaneous quantitative determination of Sudan dyes present in food products.
Journal: Food Chemistry - Volume 115, Issue 4, 15 August 2009, Pages 1556–1562