کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1188214 963483 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of stigmasterol and cholesterol oxides using atmospheric pressure chemical ionization liquid chromatography/mass spectrometry
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of stigmasterol and cholesterol oxides using atmospheric pressure chemical ionization liquid chromatography/mass spectrometry
چکیده انگلیسی

A new sensitive HPLC–MS method for the detection of sterol oxidation products was developed. The method was developed and optimized with commercially available cholesterol oxidation products. MS detection was carried out with an ion-trap mass spectrometer using atmospheric pressure chemical ionization in positive ion mode. With this new method, both primary and secondary oxidation products of cholesterol and stigmasterol, including epoxides, could be detected. Applicability of the method was investigated using thermo-oxidized (180 °C) stigmasterol as a model compound. Formation and degradation of two hydroperoxides were observed and formation of eight secondary oxidation products quantitatively determined. In conclusion, this method proved to be a powerful tool for investigating oxidation mechanisms of sterols.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 101, Issue 4, 2007, Pages 1438–1445
نویسندگان
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