کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1188888 963501 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Purification and characterization of a leucine aminopeptidase from the skeletal muscle of common carp (Cyprinus carpio)
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Purification and characterization of a leucine aminopeptidase from the skeletal muscle of common carp (Cyprinus carpio)
چکیده انگلیسی

An aminopeptidase was purified from the skeletal muscle of common carp (Cyprinus carpio) to homogeneity, with 1160-fold purification and a yield of 4.3%. The purification procedure consisted of ammonium sulfate fractionation and sequential chromatographic steps, including DEAE-Sephacel, Sephacryl S-200, hydroxyapatite, Phenyl Sepharose and Macro-Prep High Q columns. The molecular mass of the enzyme was estimated to be 105 kDa and 100 kDa by SDS-PAGE under reducing conditions and gel filtration chromatography, respectively, suggesting it to be a monomer. The enzymatic activity was optimal at 35 °C and pH 7.0. The metal-chelating agents EDTA, EGTA and 1,10-phenanthroline specifically inhibited the enzyme activity while inhibitors of other proteinases did not show much effect, indicating that it was a metalloproteinase. Furthermore, bestatin, a specific aminopeptidase inhibitor strongly inhibited its activity. Divalent cations Mn2+, Mg2+ and Ba2+ slightly enhanced the enzymatic activity, while Co2+, Cu2+, Zn2+, Ca2+ and Fe2+ inhibited the activity to different extents. In addition, a sulfhydryl reagent was necessary to maintain its activity. Substrate specificity study revealed that the purified enzyme preferentially hydrolyzed Leu-MCA, followed by Arg-MCA, Ala-MCA and Tyr-MCA and it was thus regarded as a leucine aminopeptidase (LAP, EC 3.4.11.1). The apparent Km and Vmax values of the enzyme were 4.6 μM and 9.6 μmol min−1 mg−1, respectively for substrate Leu-MCA. This is the first report concerning purification and characterization of LAP from freshwater fish.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 108, Issue 1, 1 May 2008, Pages 140–147
نویسندگان
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