کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1188985 963502 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
DPPH antioxidant assay revisited
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
DPPH antioxidant assay revisited
چکیده انگلیسی

Scavenging of DPPH free radical is the basis of a common antioxidant assay. A number of protocols have been followed for this assay resulting in variation in the results of different laboratories. We present a perspective of the protocols followed by different workers with incongruity in their results and recommend a standard procedure within the sensitivity range of spectrophotometry. Three common standard antioxidants viz. ascorbic acid, BHT and propyl gallate have been used in this study. The IC50 values for ascorbic acid and propyl gallate were 11.8 μM and 4.4 μM in methanol and 11.5 μM and 4.7 μM in buffered methanol as reaction medium, respectively. The free radical scavenging by BHT was markedly influenced by the reaction medium. The IC50 values were 60.0 μM and 9.7 μM when the reaction was done in methanol and buffered methanol, respectively.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 113, Issue 4, 15 April 2009, Pages 1202–1205
نویسندگان
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