Transglycosylation reaction of endoxylanase from Trichoderma longibrachiatum

The study of hydrolytic activities of several enzymatic preparations showed that the Glucanase GL-200 and Xylanase XL-200 enzymatic preparations from Trichoderma longibrachiatum and the Xylanase from Trichoderma viride, possessed an endoxylanase activity useful for the transfer reaction. The enzymatic synthesis of hexylxyloside and hexylxylobioside were achieved by xylose transfer, catalysed by T. longibrachiatum XL-200 xylanase crude preparation, from xylan (donor) to hexanol, with (50%, v:v) or without n-hexane used as co-solvent. Benzyl alcohol was also used as acceptor for the synthesis reaction of benzylxyloside, benzylxylobioside, and benzylxylotrioside, with the T. longibrachiatum XL-200 xylanase crude preparation and partially pure T. longibrachiatum endoxylanase. The transfer reaction due to the T. longibrachiatum endoxylanase was confirmed by the enzymatic synthesis, catalysed by T. longibrachiatum partially pure endoxylanase, of phenyl primeveroside performed from phenyl glucoside and xylan, in the presence of n-hexane. We showed that the T. longibrachiatum endoxylanase was a good tool for the synthesis of xylosyl derivatives (homo- and hetero-xylosides), by transfer reaction.