کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1190344 | 963529 | 2007 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Protein and amino acid contents in the crab, Chionoecetes opilio
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
Total protein, by the Kjeldahl method, and amino acid content, by HPLC, were determined in crab samples. Proteins were determined in the shell and in the discard meat, separately.An HPLC method was used to analyse the amino acids in dried crab shells after derivatization with phenylisothiocyanate. The resulting amino acids were chromatographed on an ODS2 column with UV detection at 254 nm. The mobile phase was a mixture of 0.14 M sodium acetate buffer, pH 6.2, containing 0.05% triethylamine and 60:40 (v/v) acetonitrile–water, at a flow rate of 0.9 ml min−1. The amino acids found at highest concentrations were arginine, lysine, glutamic acid and serine.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 103, Issue 4, 2007, Pages 1330–1336
Journal: Food Chemistry - Volume 103, Issue 4, 2007, Pages 1330–1336
نویسندگان
María Vilasoa-Martínez, Julia López-Hernández, María Asunción Lage-Yusty,