Reverse phase HPLC/DAD determination of biogenic amines as dansyl derivatives in experimental red wines

An accurate, rapid and very sensitive high-performance liquid chromatographic (HPLC) method for the determination of dansyl derivative of 11 biogenic amines, in experimental red wine samples from the crop year 2000 produced by Sicilian and French viticultures, was developed. The method involved the derivatisation with dansyl chloride followed by liquid chromatography gradient elution analysis without any other sample pre-treatment. The chromatographic system was equipped with a reversed-phase C-18 column and a DAD detector. A mobile phase of acetonitrile/water was used. The levels of biogenic amines in the samples ranged from 0.10 to 0.80 μg/ml. The HPLC method developed, showed a good linearity, sensitivity and repeatability. Recovery test ranged from 85.0% to 98.6% while detection limit was 0.01 ng/ml for all the studied compounds.