کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1191679 | 963578 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Production of trehalose by intramolecular transglucosylation of maltose catalysed by a new enzyme from Thermus thermophilus HB-8
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Thermus thermophilus HB-8 is a source of trehalose synthase (GTase), which catalyses conversion of maltose into trehalose. Specific activity of maltose transglucosylation by cell-free extracts of the bacteria was about 0.1 U mgâ1 protein and precipitation at 28% saturation of ammonium sulphate caused 3.5-fold enzyme purification. The optimum temperature for conversion of maltose into trehalose was 65 °C with about 27% of maximum activity at 85 °C. The highest GTase productivity was achieved at cultivation temperature over 60 °C and at NaCl concentration range of 0.1-0.5% (w/v). However, larger concentrations of sodium chloride in the growth media caused a remarkable decrease of GTase synthesis. The results, of ammonium sulphate fractionation and activity towards maltotriose (0.028 U mgâ1), maltotetraose (0.16 U mgâ1) and GlcαpNp (0.27 U mgâ1), show that trehalose synthase and α-glucosidase activities reside in separate protein fractions of cell-free extracts from T. thermophilus cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 96, Issue 1, May 2006, Pages 8-13
Journal: Food Chemistry - Volume 96, Issue 1, May 2006, Pages 8-13
نویسندگان
Anna ZdziebÅo, Józef Synowiecki,