A simple and rapid HPLC technique for vanillin determination in alcohol extract

This paper describes a simple and rapid HPLC technique for vanillin determination in alcohol vanilla extract. Vanillin was separated on a Nucleosil C18 column by using water and methanol (40:60) as the mobile phase and retention time was only 2.2 min. The measurements were made by using a photodiode array detector of the most adequate maximum wavelength absorbance at 231 nm. This method has been successfully applied for the determination of vanillin in some commercial extracts.