The influence of γ-irradiation on some biological activities and electrophoresis patterns of wheat grain albumin fraction

The highest increase of antiamylolytic activity against human saliva α-amylase was noted in albumin proteins of wheat grain treated by γ-radiation doses >0.5 kGy while there were decreases at radiation doses <0.5 kGy (P ⩽ 0.05). The increase of inhibition activity against hog pancreas α-amylase was noted at radiation doses 0.1 kGy (P ⩽ 0.05). γ-Radiation doses of 0.05, 0.5 and 1 kGy caused an increase of the antitryptic activity while 10 kGy decreased this activity (P ⩽ 0.05). SDS-PAGE electrophoresis patterns of albumin sample at 0.1 kGy showed 15 proteins while in the control sample only 11 bands were found. Among studied wheat albumins, the lower protein bands 5 and 8 were detected in wheat grain samples irradiated by 10 and 0.5 kGy, respectively. The differences in inhibitor activities and electrophoretic protein bands of wheat albumin from γ-irradiated wheat grain give additional evidence that these proteinaceous inhibitors against enzymes studied are different in nature.