کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1193261 | 1492304 | 2011 | 9 صفحه PDF | دانلود رایگان |
This review gives an overview and critical evaluation about modern LC-MS techniques for identification and quantification of thiol peptides and their metal complexes in seed plants, algae and fungi. In analogy to other research fields we propose the term metallo-thiolomics for the global study of the entirety of thiol peptides and their metal complexes (metallo-thiolome) aiming at the elucidation of the thiol peptide regulated metal homeostasis. Off-line and on-line approaches based on liquid chromatography (LC) and electrospray tandem mass spectrometry (ESI-MS/MS) were mainly used for identification and quantification of thiol peptide apo-forms. The complementary application of inductively coupled plasma mass spectrometry (ICP-MS) has demonstrated to be useful for the investigation of native metal–thiol peptide complexes. Furthermore, new quantification strategies via the element signal in ICP-MS will be presented. We critically discuss the impact of these approaches on the progress in plant biochemistry and highlight future trends and developments.
Figure optionsDownload high-quality image (172 K)Download as PowerPoint slideResearch highlights▶ Molecular characterization of thiol peptide regulated metal homeostasis in plants and fungi. ▶ Modern LC-MS techniques for identification and quantification of thiol peptides. ▶ Metallo-thiolome and metallo-thiolomics.
Journal: International Journal of Mass Spectrometry - Volume 307, Issues 1–3, 1 October 2011, Pages 46–54