Mass spectra of proteins and other biomolecules recorded using a handheld instrument

Proteins (myoglobin and cytochrome C), peptides (bradykinin and melittin), alkaloids in complex plant materials, and mixtures of phospholipids from a bacterial extract all give characteristic electrospray mass spectra using a handheld tandem mass spectrometer, Mini 10. The mass/charge range of the 10 kg Mini 10 was extended to m/z 2000 using resonant ion ejection at low frequency allowing analysis of proteins with molecular weights up to 17,000 Da. Fragmentation of peptides and proteins was observed.