کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
11974 770 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparison of standard surface chemistries for culturing mesenchymal stem cells prior to neural differentiation
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Comparison of standard surface chemistries for culturing mesenchymal stem cells prior to neural differentiation
چکیده انگلیسی

A critical element of any stem cell differentiation protocol is the ability to compare its effects relative to an undifferentiated population of the same cells. In an attempt to standardize pre-differentiation conditions of adult derived mesenchymal stem cells prior to neural induction experiments, we asked what is the simplest chemical surface that supports the growth and maintenance of these cells in a pre-differentiation state. Adult bone marrow-derived rat mesenchymal stem cells (BMSCs) were expanded in vitro on Permanox Lab-Tek® tissue culture treated plastic (TCP), poly-d-lysine (PDL) coated glass, PDL-laminin-1 coated glass, and untreated glass. TCP provided the best surface for maintaining morphologies generally considered to be undifferentiated, while PDL coated glass and uncoated glass provided the least suitable surfaces. Expansion of BMSCs on PDL-laminin-1 coated glass resulted in expression of nestin, a marker associated with neuronal and other progenitor cells, and therefore may confound experimental results if used as a pre-differentiation surface.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 27, Issue 24, August 2006, Pages 4333–4339
نویسندگان
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