کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1228667 | 1495204 | 2016 | 7 صفحه PDF | دانلود رایگان |
• Gradient flow injection technique has been applied for β-CD: dyes complexation study.
• Concentration gradient in FIA has been calculated via dispersion calibration.
• PCA is used for determination of stoichiometric ratios and noise reduction.
• The stability constants have been estimated by model-based multivariate methods.
• The complementary study has been done by molecular modeling.
Gradient flow injection technique-diode array spectrophotometry was applied for β-cyclodextrin (β-CD)-dye inclusion complex studies. A single injection of a small amount of mixed β-CD-dye solution (100 μl) into the carrier solution of the dye and recording the spectra gave the titration data. The mole ratio data were calculated by calibrating the dispersion pattern using a calibrator dye (rose bengal). Model-based multivariate methods were used to analyze the spectral-mole ratio data and, as a result, estimate stability constants and concentration-spectral profiles. Reliability was tested by applying this method to study the β-CD host-guest complexes with several dyes as guest molecules. Singular value decomposition (SVD) was used to select the chemical model and reduce noise. Molecular modeling provided the ability to predict the guest conformation-orientation (posing) within the cavity of β-CD and the nature of the involved interactions. Among those dyes showing observable spectral variation, the stoichiometric ratio of β-CD: dye (and log Kf) of methyl orange, fluorescein, phenol red, 4-(2-pyridylazo) resorcinol (PAR), and crystal violet were calculated to be 1:1 (4.26 ± 0.01), 1:1 (1.53 ± 0.08), 1:1 (3.11 ± 0.04), 1:1 (1.06 ± 0.12), and 2:1 (5.27 ± 0.03), respectively. Compared with the classical method of titration, this method is simple and fast and has the advantage of needing reduced human interference. Molecular modeling facilitates a better understanding of the type of interactions and conformation of guest molecules in the β-CD cavity. The details of the proposed method are discussed in this paper.
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Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 165, 5 August 2016, Pages 54–60