SERS substrates fabricated using ceramic filters for the detection of bacteria

• SERS substrates were fabricated using ceramic filters and Ag/Au colloid.
• The distribution of bacteria on the surface of the SERS substrates was homogeneous.
• SERS spectra of bacteria were obtained by rastering the laser over the sample.
• Borate buffered saline did not impact the SERS response.
• SERS can be used to differentiate bacterial species.

SERS substrates were fabricated by filtering either Ag or Au colloidal particles onto rigid, ceramic filters – onto which suspensions of bacteria were then filtered. SERS spectra of the bacteria were obtained using a Raman spectrometer that has an ‘orbital raster scan’ capability. It was shown that bacteria samples prepared in this manner were uniformly distributed onto the surface of the SERS substrate. The effect of common buffer systems on the SERS spectra was investigated and the utility of using the SERS technique for speciation of bacteria was explored.

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