Solid phase microextraction of diclofenac using molecularly imprinted polymer sorbent in hollow fiber combined with fiber optic-linear array spectrophotometry

• Preparation of a sorbent for DFC by synthesis of MIP in presence of MWCNT and its reinforcement in HF.
• Development of a MIP-HF-SPME method for preconcentration of DFC.
• Combination of MIP-HF-SPME with fiber optic-linear array spectrophotometry.
• Development of a simple, sensitive and precise method for determination of DFC in various samples.

A simple solid phase microextraction method based on molecularly imprinted polymer sorbent in the hollow fiber (MIP-HF-SPME) combined with fiber optic-linear array spectrophotometer has been applied for the extraction and determination of diclofenac in environmental and biological samples. The effects of different parameters such as pH, times of extraction, type and volume of the organic solvent, stirring rate and donor phase volume on the extraction efficiency of the diclofenac were investigated and optimized. Under the optimal conditions, the calibration graph was linear (r2 = 0.998) in the range of 3.0–85.0 μg L−1 with a detection limit of 0.7 μg L−1 for preconcentration of 25.0 mL of the sample and the relative standard deviation (n = 6) less than 5%. This method was applied successfully for the extraction and determination of diclofenac in different matrices (water, urine and plasma) and accuracy was examined through the recovery experiments.

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